IEEE BioCAS 2021
Filterless TRF Reader with CMOS Sensor ASIC for Lateral Flow Immunoassays. Alexander Hofmann1, Peggy Reich1, Martin Grabmann1, Georg Gläser1, Max Trübenbach2, Alexander Rolapp1, Marco Reinhard1, Friedrich Scholz2, and Eric Schäfer1
1IMMS, 2Senova Gesellschaft für Biowissenschaft und Technik mbH
Abstract — In this paper, we present a filterless time-resolved fluorescence (TRF) measurement system with an optoelectronic CMOS sensor for highly sensitive readout of lateral flow assay (LFA) test strips. An application-specific integrated circuit (ASIC) was designed in a standard 0.35-μm CMOS technology and includes a 5 × 5 CMOS sensor array. Each sensor pixel contains a 100 μm × 100 μm photodiode acting as signal transducer. For the precise control of the measurement sequence and its timing regime, a microcode engine (MCE) has been realized in a field-programmable gate array (FPGA). An active LED discharge concept was implemented to prevent long afterglow of the light source after it is turned off. To characterize the TRF reader with respect to uniform and reproducible signal intensities, suitable europium (III) [Eu 3+] chelate particles were used as fluorescence standards. Different concentrations of europium-based nanoparticles (NP) on test strips were quantified with the TRF reader. Furthermore, we compared the developed TRF reader to a commercial colorimetric LFA reader system using human C-reactive protein (hCRP) as model analyte. The CMOS sensor ASIC based filterless TRF reader showed a detection dynamic range (DDR) of 4 orders of magnitude with a LoD of 0.2 ng/ml for the quantification of CRP, which was 3.5 times better than the LoD of the commercial LFA reader.
Dipl.-Hdl. Dipl.-Des. Beate Hövelmans
Head of Corporate Communications
beate.hoevelmans(at)imms.de+49 (0) 3677 874 93 13
Beate Hövelmans is responsible for the text and image editorial work on this website, for the social media presence of IMMS on LinkedIn and YouTube, the annual reports, for press and media relations with regional and specialist media and other communication formats. She provides texts, photographs and video material for your reporting on IMMS, arranges contacts for interviews and is the contact person for events.